| 1. | The blood of each rabbit was collected at days 0 , 7 , 14 , 2l , 30 . the antibody titers were evaluated by k - agglutination test . the results showed that lower agglutination titers was observed at day 7 and up to higher levels over 8000at day 21 induced by the vaccine of recombinant fused protein 将融合基因工程菌株在营养肉汤中培养,离心后上清用0 . 1mmgcl _ 2沉淀,离心,提取表达产物。经sds - page电泳证明表达产物为纤毛蛋白与il - 2的融合蛋白。 |